Parts | Days | Selection | Search | Updates | Downloads | Help
BP: Fachverband Biologische Physik
BP 15: Tissue Mechanics II
BP 15.5: Talk
Wednesday, March 29, 2023, 11:45–12:00, BAR Schö
Capturing the mechanosensitivity of cell proliferation in models of epithelium — •Maxime Hubert1, Kevin Höllring1, Lovro Nuić2, Luka Rogić2, Sara Kaliman1, Simone Gehrer1, Florian Rehfeldt3,4, and Ana-Sunčana Smith1,2 — 1FAU Erlangen-Nürnberg, Erlangen, Germany — 2Ruđer Bošković Institute, Zagreb, Croatia — 3University of Göttingen, Göttingen, Germany — 4University of Bayreuth, Bayreuth, Germany
The proliferation of epithelial cells, the process of cell growth and cell division, is affected by the mechanical properties of the surrounding environment. While an extensive literature covers single cell mechanoresponse, information about tissue-wide mechanoresponse are scarce. It is only known that high cell density restricts proliferation and eventually leads to homeostasis. In this presentation, we aim at completing the existing literature by addressing the role of both cell density and extracellular stiffness in the proliferation of cells in epithalial monolayer. Using MDCK-II epithelial tissues stained with EdU we are able to measure the fraction of dividing cells at a given cell density and quantify mechanoresponse. We build a cell-level theory of proliferation based on a two-population description which is compared successfully to the experiments and implemented into simulations. Using experiments and simulations, we also address the role of proliferation in the large scale growth of tissues. A tissue-scale theory of epithelial growth based on the cell-level findings is finally presented. This work provides a first step towards a complete description of proliferation at the tissue scale and its influence on its compartmentalization.