SKM 2023 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 15: Tissue Mechanics II

BP 15.6: Vortrag

Mittwoch, 29. März 2023, 12:00–12:15, BAR Schö

Quantitative 3D live-imaging of self-organisation in embryonic organoids — •Valentin Dunsing, Sham Tlili, Claire Chardès, Léo Guignard, and Pierre-François Lenne — IBDM & CENTURI, Aix-Marseille University/ CNRS, Marseille, France

The emergence of asymmetries within a mass of equivalent cells is the starting event in the development of embryos, resulting in the formation of the main body axes. Despite its fundamental role, the mechanisms that induce symmetry breaking remain largely unknown because they are difficult to probe in vivo, particularly in mammalian embryos. A promising in vitro model to study such mechanisms are embryonic organoids, which undergo gastrulation-like movements similar to those observed in embryos. We aim to use live imaging to disentangle the interplay between signaling, cell differentiation and mechanics underlying self-organized symmetry breaking. Currently available imaging platforms are limited to low-throughput 3D or high-throughput 2D imaging. To overcome this limitation, we establish multi-view single-objective lightsheet microscopy, allowing us to image tens of organoids over hours to days with cellular resolution and sufficient temporal sampling to track cells in 3D. We present ongoing efforts using deep learning based segmentation and quantitative image analysis to correlate cellular dynamics and rearrangements with the expression of key differentiation markers during polarization of aggregates. We thereby analyze how spatially localized expression domains and collective cell movements establish symmetry breaking. Finally, we analyze the variability of spatiotemporal patterns across multiple specimen.