SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 21: Bioimaging
BP 21.11: Vortrag
Donnerstag, 30. März 2023, 12:30–12:45, BAR Schö
Mechanical and electrophysiological recordings of neural organoids — Elijah Shelton1, Paulina Wysmolek2, Filippo Kiessler1, Achim Brinkop1, Sebastian Willenberg1, Michael Frischmann1, and •Friedhelm Serwane1,2,3 — 1Faculty of Physics & CeNS, LMU Munich, Germany — 2MPI for Medical Research, Heidelberg, Germany — 3Munich Cluster for Systems Neurology, Munich, Germany
Stem-cell derived organoids have made the exploration of neuronal network function accessible in vitro and are now allowing disease modelling. Both biochemical and mechanical signals, such as the elastic modulus, modulate the underlying behaviour of neurons to connect to networks. My group is developing tools for the mechanical and electrophysiological characterization of neuronal organoids. I will present a minimal-complexity setup for 3D imaging of their network activity (Wysmolek et al., Sci Rep 12, 20420, 2022). To extract Ca-signals we combine a lightsheet microscope as an add-on to a standard inverted microscope with computational tools. We created a 3D connectivity map by imaging spontaneous activity. As a next step, we apply statistical models to characterize the network behaviour. Changes in the tissue mechanical properties are one biophysical hallmark of tumour formation in vivo. We map the mechanical properties of tumor-forming cerebral organoids using ferrofluid droplets as mechanical actuators. Our measurements performed in neural organoids could inform researchers about the interaction between mechanics and function in the central nervous system.