SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 21: Bioimaging
BP 21.12: Vortrag
Donnerstag, 30. März 2023, 12:45–13:00, BAR Schö
Multi-scale X-Ray phase contrast tomography from the whole cochlea to single cells — •Jannis Justus Schaeper1, Christoph Kampshoff2, Bettina Wolf2, Daniel Keppeler2, Tobias Moser2, and Tim Salditt1 — 1Institut für Röntgenphysik, Georg-August-Universität Göttingen — 2InnerEarLab, Universitätsmedizin Göttingen
The cochlea is the receptor organ of the inner ear which transduces sound into neuronal activity. Both fundamental aspects of signal transduction and neuro-physiology as well as biomedical research (implant technology, hearing loss and disorders) require 3D imaging techniques capable to quantify the micro-anatomy (1).
We present multi-scale 3D imaging of small-animal cochleae by X-ray phase-contrast tomography (XPCT) using both synchrotron radiation (SR) and lab µ-CT to assess the morphology of the cochlea, orientation of cochlear implants (CIs), and the number and density of spiral ganglion neurons (SGNs). Due to optimization in sample preparation, image acquisition and phase retrieval we achieve high contrast for unstained soft tissue. Without extensive sample preparation, shape and volume of every SGN in the entire organ can be identified. In the high-resolution PC-CT, and in the parallel beam, we reach cellular resolution in the organ of Corti. Lab µ-CT is suitable to analyze cochlear morphology and to assess the correct positioning of CIs and resulting (non-)optimal signal transduction.
(1) Keppeler et al. (2021), PNAS 118(18), e2014472118 (2) Schaeper et al. (2022), Proc. SPIE 12242