SKM 2023 – scientific programme
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BP: Fachverband Biologische Physik
BP 21: Bioimaging
BP 21.1: Talk
Thursday, March 30, 2023, 09:30–09:45, BAR Schö
Imaging DNA-Origami with Low Energy Electron Holography — •Moritz Edte1, Hannah Ochner1, Luigi Malavolti1, and Klaus Kern1,2 — 1Max-Planck-Institute for Solid State Research, Stuttgart, Germany — 2École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
Our low-energy electron holography (LEEH) approach makes use of a coherent low-energy electron wave (energies in the range of 50 to 150 eV) for imaging biomolecules with high contrast [1]. Hence, the method allows true single-molecule imaging of large three-dimensional molecules and can map conformational variability of flexible molecules [2]. I will show LEEH measurements of individual DNA-Origami molecules on single layer graphene, demonstrating that LEEH is capable of imaging this class of molecules without introducing perceptible structural changes on the time scale of the measurements and on the spatial scale defined by our current resolution limit.
[1] J.-N. Longchamp, et al., PNAS, 2017, 114, 1474-1479. [2] H. Ochner, et al., PNAS, 2021, 118, e2112651118