SKM 2023 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 21: Bioimaging

BP 21.6: Vortrag

Donnerstag, 30. März 2023, 10:45–11:00, BAR Schö

Adaptive optics in Confocal microscopy and Fluorescence correlation spectroscopy — •Julius Trautmann, Philipp Kellner, and Christian Eggeling — Institute for Applied Optics and Biophysics, Friedrich-Schiller University Jena, Philosophenweg 7, 07743 Jena

For more than three decades adaptive optics have been widely used in astronomical applications, but only in recent years it has established itself as an important feature for high resolution microscopy. The abillity to correct for optical aberrations can be useful for any kind of setup but it proved particularly useful when imaging samples with inhomogeneous refractive index structures such as cells and especially cell tissue.

The most established adaptive optic elements include deformable mirrors (DMs) and spatial light modulators (SLMs) which can dynamically correct for aberrations.

This talk will cover the basic idea of including a deformable mirror (DM) in a confocal microscope within a fluorescence correlation spectroscopy (FCS) setup. A comparison of placing the deformable mirror in the excitation or detection beam path will take place.