SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 25: Cell Mechanics II
BP 25.3: Vortrag
Donnerstag, 30. März 2023, 15:30–15:45, BAR Schö
T-cell migration: Improving searching efficiency by targeting Microtubules — •Galia Montalvo1,2,3, Bin Qu3,4, and Franziska Lautenschläger1,2,3 — 1University of Saarland, Department of Experimental Physics — 2University of Saarland, Center for Biophysics — 3Biophysics, Center for Integrative Physiology and Molecular Medicine (CIPMM), School of Medicine — 4Leibniz Institute for New Materials
Cytotoxic T lymphocytes (CTLs) are the key players in the adaptive immune system to eliminate tumor cells. Proper mobility in three-dimensional environments is a prerequisite for CTLs to execute their killing function and the cytoskeleton plays a central role in CTL migration in 3D. In dense matrices, migration and the consequent killing efficiency of CTLs is greatly impaired. Interestingly, we observed that in 3D matrices, CTLs go through narrow quasi-1D channels. Manufactured micro-channels are a recently emerged promising tool with well-defined parameters to investigate cell migration in 1D. In this project, we characterize MTs as a major cytoskeletal component regulating the migration of CTLs in micro-fabricated environments and in dense collagen matrices. To this end, we expose CTLs to MTs disturbing drugs. We studied the mechanics of treated cells and described the role of actin in the observed stiffness increase after MT depolymerization. Our main conclusion is that searching efficiency and killing capacity of CTLs is enhanced after MT depolymerization through stiffness increase.