SKM 2023 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 25: Cell Mechanics II

BP 25.4: Vortrag

Donnerstag, 30. März 2023, 15:45–16:00, BAR Schö

Global membrane tension is independent of polyacrylamide substrate stiffness — •Eva Kreysing¹, Jeffrey McHugh^{1, 2}, Sarah Foster^{1, 3}, Kurt Andresen⁴, Ryan Greenhalgh¹, Eva Pillai^{1, 5}, Andrea Dimitrcopoulus¹, Ulrich Keyser¹, and Kristian Franze^{1, 6} — ¹University of Cambridge, UK — ²Collège de France, Paris — ³University of Tübingen; Germany — ⁴Gettysburg College, USA — ⁵EMBL Heidelberg, Germany — ⁶University Erlangen/Nürnberg, Germany

Cellular processes such as cell migration or axonal pathfinding have been shown to depend strongly on mechanical properties of the cell*s environment such as stiffness. It has been shown that Piezo1 is one of the mechanosensitive ion channels that contributes to such behaviour. It is hypothesised that Piezo1 is activated by changes in membrane tension. This raises the question whether membrane tension is influenced by stiffness of the cell*s environment. We measured membrane tension as a function of substrate stiffness in different cell types with optical tweezers. To our surprise, we found that global membrane tension is independent of substrate stiffness in the physiological range. However, we found strong differences between membrane tension on compliant substrates and glass substrates. To explain these observations, this work introduces a toy model for substrate, membrane and cortex interaction.