SKM 2023 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 26: Focus Session mRNA Physics

BP 26.2: Vortrag

Donnerstag, 30. März 2023, 15:30–15:45, TOE 317

Decomposition of ensemble fluorescence signals from translation experiments and simulations — •Nadin Haase, Simon Christ, and Sophia Rudorf — Institute of Cell Biology and Biophysics, Leibniz University Hannover, Germany

Proteins are major components of cells and perform all kinds of tasks essential for survival. Those parts of the DNA that contain blueprints for the synthesis of proteins are transcribed to messenger RNA (mRNA). Complex biomolecular machines called ribosomes translate the mRNA by assembling the corresponding building blocks of proteins, the amino acids. This process can be studied by in-vitro ensemble experiments through monitoring time-dependent output signals such as fluorescence time traces, where the ensemble signal is a superposition of the individual signals from all molecules in the reaction. However, translation is a stochastic process and thus even for initially synchronized reactions the ensemble signal may not reflect the features in the individual signals, especially on longer time scales. Here we present an approach to decompose the ensemble signal to reveal hidden information about the individual steps of the translation process. We explore the limits of this method and study the conditions under which a meaningful solution can be gathered.