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BP: Fachverband Biologische Physik

BP 28: Poster Session II

BP 28.11: Poster

Donnerstag, 30. März 2023, 18:00–20:00, P2/EG

3D light-sheet microscopy of contracting skeletal muscle tissue — •Laura Strampe, Arne Hofemeier, Paul Maier, Jan Huisken, and Timo Betz — Third Institute of Physics - Biophysics, Georg August Universität, Göttingen

Skeletal muscle makes up the majority of human muscle tissue. Its contraction allows for controlled movements of the body, as well as being essential for maintaining posture. To understand the global and local forces at play during such contractions, occurring on the time scale of seconds, a 3D high-speed, high-resolution imaging method is necessary. We present a protocol for raising biomimetic muscle tissue anchored between two flexible mm-sized post. A full contraction cycle can be recorded through the synchronization of periodic electric stimuli and image acquisition using a custom build Flamingo light-sheet microscope. This enables us to study a wide range of questions including the influence of local contractile forces on muscle stem cell activation and contractile sarcomere dynamics.