SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 28: Poster Session II
BP 28.13: Poster
Donnerstag, 30. März 2023, 18:00–20:00, P2/EG
Imaging of vital mitochondria using Scanning Ion Conductance Microscopy (SICM) and electron microscopy methods — •Eric Lieberwirth1, Christian Völkner1, Regina Lange1, Anja Schaeper2, Magdalena Otte2, Marcus Frank3, Kevin Oldenburg4, Ingo Barke1, Simone Baltrusch2, and Sylvia Speller1 — 1University of Rostock, Institute of Physics — 2Rostock University Medical Center, Institute for Medical Biochemistry and Molecular Biology — 3Rostock University Medical Center, Medical Biology and Electron Microscopy Center — 4University of Rostock, Center for Interdisciplinary Electron Microscopy MV
Mitochondria are enclosed by a double membrane. While the inner membrane with many insertions creates a large surface for the respiratory chain complexes, the outer one mediates fusion, fission and degradation of the organelles in the network. In this process, pole-like interaction sites develop, which impact the surface of the outer membrane. Scanning Ion Conductance Microscopy (SICM) allows the outer membrane of immobilized vital mitochondria to be measured in a buffer solution with a spatial resolution of about 50 nm and a height resolution of a few nanometers. The outer membrane shows dynamic height fluctuations as well as spatial height variations depending on the medium. Transmembrane proteins can be made visible when labelled with nanoscopic gold particles. A complementary study of SICM topographies, Scanning Electron Microscopy (SEM) and in situ liquid Transmission Electron Microscopy (TEM) images gives insight into the spatial distribution of internal and membrane-bound structures.