SKM 2023 – scientific programme
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BP: Fachverband Biologische Physik
BP 28: Poster Session II
BP 28.18: Poster
Thursday, March 30, 2023, 18:00–20:00, P2/EG
Shaping the embyo: blastoderm stress maps reveal early mechanical symmetry breaking — •Alejandro Jurado Jiménez, Leon Lettermann, Bernhard Wallmeyer, and Timo Betz — Drittes Physikalisches Institut, Georg-August-Universität Göttingen
In this work we present a hydrodynamical analysis of early Zebrafish development which aims to understand the mechanical state of the tissue leading to its first symmetry breaking during epiboly: the shield formation. A full mechanical characterization of the blastoderm is achieved using a combination of Light-Sheet microscopy and state-of-the-art cell tracking of the cells nuclei, viscosity measurements and polyacrylamide beads as force sensors. The extraction of stress maps in the tissue is possible thanks to a custom-made software for the analysis of the bead deformation, which is presented here a versatile tool for similar stress analyses in other biological samples.
Our experimental analysis of the mechanical state of the embryo is supported and expanded by a model-driven extraction of the stress fields using NeuronalODEs. The NODEs system only necessitates the velocity field on the blastoderm to solve the hydrodynamic problem, optimizing up to 105 parameters and retrieving a full dynamical description of the embryo. Both the experimental and numerical analyses expose a stress asymmetry prior and during the shield formation, from which we can learn more about the mechanical origin of the first embryonal symmetry breaking.