SKM 2023 – scientific programme

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BP: Fachverband Biologische Physik

BP 28: Poster Session II

BP 28.25: Poster

Thursday, March 30, 2023, 18:00–20:00, P2/EG

Interactions between synaptic vesicles and cytoskeletal filaments — •Tiago Mimoso, Tizian Schmidt, and Sarah Köster — Institute for X-Ray Physics, University of Göttingen, Germany

Signal transmission of neurons occurs both electrically and chemically. The chemical signal is transported by synaptic vesicles(SVs) from one cell to another via the synaptic cleft to the adjacent neuron. Thus, these SVs are found in the synapse, within the so-called synaptic bouton. Here, the SVs are surrounded by cytoskeletal filaments, including dynamic microtubules that undergo rapid assembly and disassembly. There are studies that suggest an interactions between SVs and the cytoskeletal filaments, however the exact mechanisms remain unknown. Therefore, we now ask the question of whether SVs and microtubules interact and what influence the presence of SVs has on the growth rate, disassembly rate, catastrophe frequency and rescue frequency of the microtubules. We employ a reconstituted in vitro system and image the dynamic microtubules by total internal reflection fluorescence (TIRF) microscopy. We use a novel and fast data analysis technique based on a neural network to segment the microtubules in the microscopy pictures. This method provides the advantage of being applicable also curved microtubules and yields results that are comparable to the commonly used manual analysis method.