SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 28: Poster Session II
BP 28.3: Poster
Donnerstag, 30. März 2023, 18:00–20:00, P2/EG
Comparative investigation of differently prepared porcine retinal pigment ephitelium (RPE) cells using mid-IR photo-induced force microscopy (PiF-IR) — •Jesvin Joseph1,2, Robin Schneider1, Rowena Simon3, and Daniela Täuber1,2,4 — 1Heintzmann Lab, Leibniz Institute of Photonic Technology, Jena — 2Institute of Physical Chemistry & Abbe Center of Photonics, Friedrich-Schiller-University Jena — 3Department of Ophthalmology, University Hospital Jena — 4Institute of Solid State Physics, Friedrich Schiller University Jena, Germany
Age-related loss of sight caused by macular degeneration (AMD) is monitored in clinical health care using fundus autofluorescence, which investigates variations in the autofluorescence signal stemming from the retinal pigment epithelium (RPE)[1]. Nanolocal chemical characterization of RPE cells and pigment granules could deepen the understanding of involved cellular processes. Nano-infrared imaging methods can provide chemical information at a lateral resolution below 10 nm[2]. We used PiF-IR to investigate externally grown porcine RPE cells, which were dried in varied conditions. First results show spectral variations obtained from cells dried at room temperature and at 37°C. We compare these spectra to PiF-IR and conventional FTIR spectra obtained from plain retinal pigment chemicals. Funding by Profil Line Light, project pintXsum, FSU Jena, is acknowledged. [1] Schultz, R., Schwanengel, L., Klemm, M., Meller, D. & Hammer, M. Acta Ophthalmologica (2021). [2] Luo, X. Xue, Y.; Wu, J.; Cai, W.; Täuber, et al. Applied Physics Letters DOI: 10.1063/5.0128850.