SKM 2023 – scientific programme
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BP: Fachverband Biologische Physik
BP 28: Poster Session II
BP 28.41: Poster
Thursday, March 30, 2023, 18:00–20:00, P2/EG
FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking — Lara Hauke1, Andreas Primeßnig1, •Emese Zavodszky1, and Florian Rehfeldt2 — 1Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Germany — 2Experimental Physics I, University of Bayreuth, Germany
Cytoskeletal pattern formation and structural dynamics are key to a variety of biological functions and a detailed and quantitative analysis yields insight into finely tuned and well-balanced homeostasis and potential pathological alterations. High content life cell imaging of fluorescently labeled cytoskeletal elements under physiological conditions is nowadays state-of-the-art and can record time lapse data for detailed experimental studies. However, systematic quantification of structures and in particular the dynamics (i.e. frame-to-frame tracking) are essential. Here, an unbiased, quantitative, and robust analysis workflow that can be highly automatized is needed. For this purpose we upgraded and expanded our fiber detection algorithm FilamentSensor [1] to the FilamentSensor 2.0 [2] toolbox, allowing for automatic detection and segmentation of fibrous structures and the extraction of relevant data (center of mass, length, width, orientation, curvature) in real-time as well as tracking of these objects over time and cell event monitoring. Furthermore, we offer the Focal Adhesion Filament Cross-correlation Kit (FAFCK) [3] for automated correlation with point-like structures. [1] B. Eltzner, et al., PLoS One, 2015 [2] L. Hauke, et al., PLoS One, under review [3] L. Hauke, et al., PLoS One, 2021