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BP: Fachverband Biologische Physik

BP 28: Poster Session II

BP 28.41: Poster

Donnerstag, 30. März 2023, 18:00–20:00, P2/EG

FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking — Lara Hauke¹, Andreas Primeßnig¹, •Emese Zavodszky¹, and Florian Rehfeldt² — ¹Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Germany — ²Experimental Physics I, University of Bayreuth, Germany

Cytoskeletal pattern formation and structural dynamics are key to a variety of biological functions and a detailed and quantitative analysis yields insight into finely tuned and well-balanced homeostasis and potential pathological alterations. High content life cell imaging of fluorescently labeled cytoskeletal elements under physiological conditions is nowadays state-of-the-art and can record time lapse data for detailed experimental studies. However, systematic quantification of structures and in particular the dynamics (i.e. frame-to-frame tracking) are essential. Here, an unbiased, quantitative, and robust analysis workflow that can be highly automatized is needed. For this purpose we upgraded and expanded our fiber detection algorithm FilamentSensor [1] to the FilamentSensor 2.0 [2] toolbox, allowing for automatic detection and segmentation of fibrous structures and the extraction of relevant data (center of mass, length, width, orientation, curvature) in real-time as well as tracking of these objects over time and cell event monitoring. Furthermore, we offer the Focal Adhesion Filament Cross-correlation Kit (FAFCK) [3] for automated correlation with point-like structures. [1] B. Eltzner, et al., PLoS One, 2015 [2] L. Hauke, et al., PLoS One, under review [3] L. Hauke, et al., PLoS One, 2021