SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 28: Poster Session II
BP 28.49: Poster
Donnerstag, 30. März 2023, 18:00–20:00, P2/EG
On multistability and constitutive relations of cell motion on Fibronectin lanes — •Johannes Clemens Julius Heyn1, Behnam Amiri2, Christoph Schreiber1, Martin Falcke2,3, and Joachim Oskar Rädler1 — 1Ludwig-Maximilians-Universität München — 2Max Delbrück Center for Molecular Medicine in the Helmholtz Association — 3Humboldt Universität zu Berlin
Migration of eukaryotic cells is a fundamental process for embryonic development, wound healing, immune responses, and tumour metastasis. Experiments on 2d migration show a broad spectrum of morphodynamic features for many cell types. Cells exhibit distinct motile states: They are spread or moving and either steady or oscillatory and they display spontaneous transition between those states. Here, we present a study of the motion of MDA-MB-231 cells on 1d Fibronectin (FN) microlanes and group the migratory behaviour into four discrete states. A high-throughput setup allows to quantitatively analyse state transitions for a broad range of FN densities. We develop a biophysical model based on the force balance at the protrusion edge, the noisy clutch of retrograde flow and a response function to integrin signalling. The model reproduces cell states, characteristics of oscillations and state probabilities in very good agreement with our experimental data. The statistics of trajectories and theory suggests an adhesion related mechanism that not only explains multistability but also the well-known biphasic adhesion-velocity relation and the universal correlation between speed and persistence (UCSP).