SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 28: Poster Session II
BP 28.53: Poster
Donnerstag, 30. März 2023, 18:00–20:00, P2/EG
From Shape to Function of Sampling Resident Tissue Macrophages — •Miriam Schnitzerlein1, Anja Wegner2, Stefan Uderhardt2, and Vasily Zaburdaev1 — 1Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg and Max-Planck-Zentrum für Physik und Medizin, Erlangen, Germany — 2Department of Internal Medicine 3 - Rheumatology and Immunology, Friedrich-Alexander-Universität Erlangen-Nürnberg und Universitätsklinikum Erlangen, Germany
Mammalian tissues are permanently subjected to various stresses - be it pathogens, dead cells and related waste products or injuries like micro-lesions - which have to be resolved properly to prevent inflam- mations and maintain tissue homeostasis. To detect such incidents, sessile resident-tissue macrophages (RTMs) persistently sample their surroundings by seemingly random extension and retraction of their protrusions. Quantifying these sampling dynamics over time and comparing RTM behaviour under different conditions can uncover certain patterns or strategies in RTM sampling, which will then help us to understand how RTMs ensure tissue homeostasis. In this project, we have employed a high-resolution intravital imaging protocol to generate movies of RTM sampling dynamics in vivo. Next we have built an image processing pipeline to assess cell dynamics via its shape, the curvature and displacement of the cell membrane as well as the movement of the cell protrusions over time. Such detailed measurements enable differentiating physiological states of RTMs, and will help to build a quantitative mathematical model for RTM protrusion dynamics.