SKM 2023 – scientific programme
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BP: Fachverband Biologische Physik
BP 28: Poster Session II
BP 28.9: Poster
Thursday, March 30, 2023, 18:00–20:00, P2/EG
Quantifying Molecular Mobility, Abundance and Interactions by Fluorescence Correlation Spectroscopy — •Jana Sütterlin1, Katharina Reglinski1,2,3, Francisco Páez Larios1,2, and Christian Eggeling1,2 — 1Institut für angewandte Optik und Biophysik, Friedrich-Schiller Universität Jena, Jena, Deutschland — 2Leibnitz-Institut für photonische Technologien e.V., Jena, Deutschland — 3Universitätsklinikung Jena, Jena, Deutschland
Since many biological functions rely on molecular interactions, knowledge about molecular mobility and diffusional processes are key to understand cellular signalling. To gain the desired insights, it is of utmost importance to develop and apply live-cell compatible approaches for diffusional investigations. Fluorescence Correlation Spectroscopy (FCS) is a powerful technique that enables the quantification of these dynamics.
This poster will provide an outline on how to utilise parameters acquired from FCS and dual-colour Fluorescence Cross-Correlation Spectroscopy (DC-FCCS) to quantify diffusion characteristics of particles in aqueous environments. This way, an examination of diffusional behaviour influenced by various binding conditions can be carried out.
FCS study about the peroxisomal import receptor PEX5 in live HEK cells is shown to elucidate its molecular interaction dynamics within the cytosol. Thereby the potential of FCS is exploited, highlighting its non-invasive, live-cell compatible properties. Ensuring proper function of organelles and cells, PEX5 transports cargo proteins featuring a targeting sequence, which need to be imported into peroxisomes.