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BP: Fachverband Biologische Physik
BP 4: Tissue Mechanics I
BP 4.2: Talk
Monday, March 27, 2023, 15:15–15:30, BAR Schö
Hydraulic and osmotic control of lumen coarsening — •Mathieu Le Verge Serandour1, 2 and Hervé Turlier2 — 1School of Natural Sciences, Technical University of Munich, Germany — 2Center for Interdisciplinary Research in Biology, Collège de France, PSL Research University, Paris, France
The blastocoel formation is a keystone in the morphogenesis of the pre-implantation mammalian embryo, yet the physical mechanism for its emergence remained unclear. The blastocoel is a fluid-filled cavity (or lumen) that positions the first axis of symmetry of the embryo. We showed that, in the mouse embryo, the blastocoel results from micron-sized lumens, nucleating at the adhesive basolateral side of embryonic cells and coarsening in a process akin to Ostwald ripening. We investigate the collective dynamics of a one-dimensional chain of lumens as a minimal model for the blastocoel formation, taking the osmotic effects into account. We include the permeation of water and osmolyte through the cellular membrane. We show that the coarsening of the chain is reminiscent of dewetting films, with a scaling law for the number of lumens controlled by a screening length associated with water permeation, while the influence of osmotic inhomogeneities remains limited. Finally, we consider active osmolyte pumping that may rescue the chain from collapse. We find a new scaling law controlled by active pumping emerging from the coalescence of lumens, which may also direct the position of the final lumen.