DPG Phi
Verhandlungen
Verhandlungen
DPG

SKM 2023 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

KFM: Fachverband Kristalline Festkörper und deren Mikrostruktur

KFM 8: Diamond and related dielectric materials

KFM 8.5: Vortrag

Mittwoch, 29. März 2023, 15:20–15:40, POT 51

Enhanced protein immobilization by nanostructuring of UNCD surface — •Daniel Merker1, Daniela Bertinetti2, Rolf Merz3, Michael Kopnarski3, Friedrich W. Herberg2, Johann P. Reithmaier1, and Cyril Popov11Institute of Nanostructure Technologies and Analytics (INA), Center for Interdisciplinary Nanostructure Science and Technology (CINSaT), University of Kassel, Germany — 2Department of Biochemistry, CINSaT, University of Kassel, Germany — 3Institut für Oberflächen- und Schichtanalytik GmbH, Kaiserslautern, Germany

Investigations of molecular mechanisms related to the formation of the inner clock require detection of coupling factors, e.g. secreted neuropeptides, that mediate synchronization. In this work we study the application of ultrananocrystalline diamond (UNCD) as biosensor for detection of such coupling factors. The crucial part is the feasibility of protein immobilization on UNCD surfaces. From all investigated coupling routs only photochemical attachment of alkenes gave acceptable results when the green fluorescent protein (GFP) was immobilized. A process to create nanostructures was developed to increase the effective surface for protein immobilization. Both functionalization and structuring have beneficial effects on immobilization performance, but especially in combination the immobilization efficacy increases significantly. Lastly, the immobilization of different binding proteins against GFP - antibodies, nanobodies, DARPins - was investigated, with the last two showing promising results for high specificity and affinity capture of target molecules in a future UNCD biosensor.

100% | Mobil-Ansicht | English Version | Kontakt/Impressum/Datenschutz
DPG-Physik > DPG-Verhandlungen > 2023 > SKM