SKM 2023 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 26: Poster: New Methods
O 26.1: Poster
Montag, 27. März 2023, 18:00–20:00, P2/EG
Instrumentation for high-resolution biomolecule imaging enabled by electrospray ion beam deposition (ES-IBD) — •Lukas Eriksson, Tim Esser, Márkó Grabarics, Paul Fremdling, and Stephan Rauschenbach — University of Oxford, Department of Chemistry, United Kingdom
Elucidating the structure of biomolecules is essential to understanding their function. Direct imaging with cryogenic electron microscopy (cryo-EM) or scanning probe microscopy (SPM) is a powerful approach for finding molecular structure. However, sample preparation can be a major challenge: either very time- and resource-intensive or incompatible with the vacuum environment required by the imaging method.
Here, we explore preparative mass spectrometry as an alternative workflow towards structural elucidation of biomolecules. A novel, custom-built deposition stage extending a commercial mass spectrometer allows for the mass-filtered, soft-landed deposition of a wide mass range of target molecules (m = 100 - 106 amu) onto various surfaces, including cryo-EM grids and metal crystals for SPM.[1] This requires extensive control over conditions such as pressure, temperature, ion trajectories, sample surfaces, and sample transfer to obtain clean, chemically pure samples of the desired species in the right (i.e. native) configuration.
[1] Paul Fremdling, Tim K. Esser, Bodhisattwa Saha, Alexander A. Makarov, Kyle L. Fort, Maria Reinhardt-Szyba, Joseph Gault, and Stephan Rauschenbach: A Preparative Mass Spectrometer to Deposit Intact Large Native Protein Complexes. ACS nano 16 (2022)