SKM 2023 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 66: Poster: Scanning Probe Microscopy with Quartz Sensors
O 66.4: Poster
Mittwoch, 29. März 2023, 18:00–20:00, P2/EG
Atomic-scale imaging of individual cyclic oligosaccharides with non-contact atomic force microscopy — •Márkó Grabarics1,3, Benjamin Mallada Faes2,3, Alejandro Jiménez-Martín2, Pavel Jelínek2, Bruno de la Torre2, and Stephan Rauschenbach1 — 1University of Oxford, Department of Chemistry, United Kingdom — 2Institute of Physics of Czech Academy of Sciences, Czech Republic — 3These authors contributed equally.
Carbohydrates, also referred to as saccharides, are a major class of biopolymers that are essential to all known living organisms. While important in a range of biological processes, the structural complexity of carbohydrates poses a challenge to conventional analytical techinques, which often fail to provide unambiguous structural assignment.
We aim to overcome this challenge by imaging single, surface-adsorbed carbohydrate molecules using high-resolution SPM. For these proof-of-concept experiments, β-cyclodextrin was chosen as model compound, a cyclic oligosaccharide consisting of seven glucose units linked together via α-1,4-glycosidic linkages. Incompatible with thermal evaporation, the molecules were deposited in ultrahigh vacuum onto close-packed noble metal surfaces using electrospray deposition. STM and non-contact AFM with CO-functionalized tips revealed two distinct adsorption geometries, with the individual glucose units clearly resolved within the macrocycles.
Our results demonstrate the potential of high-resolution SPM for the structural characterization of carbohydrates, opening up novel ways for the analysis of this important class of biomolecules.