SKM 2023 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 66: Poster: Scanning Probe Microscopy with Quartz Sensors
O 66.5: Poster
Mittwoch, 29. März 2023, 18:00–20:00, P2/EG
observation and definition of lipid raft in live MCF-7 cell by AFM — •Hsiang-Ling Chuang1, Yu-Chen Fa2, Chun-Hsien Chen1, Lli-Chen Wu3, and Ja-An Ho1,2 — 1department of chemistry, National Taiwan University, Taiwan 10617. — 2department of biochemical science and technology, National Taiwan University, Taiwan 10617. — 3department of applied chemistry, National Chi Nan University, Nantou, Taiwan 54561.
Lipid rafts are composed of cholesterol, sphingolipid, and proteins. Previous studies indicated that resveratrol and fibrinogen can bind to the receptor on alpha-v beta-3 integrin. Through the protein-lipid or protein-protein interactions, drug-bound integrins will aggregate into larger raft blocks with unbound integrins. Due to the limitation of optical microscopy and complicated sample preparation, the techniques on lipid rafts observation have shortages of real-time and original state information. In this study, we explore the application of in-situ atomic force microscope (AFM) for the observation of lipid rafts on the cell surface and the response upon the administration of chemicals in real time. We obtained AFM images of morphology and stiffness of live breast cancer cells (MCF-7) in phosphate buffered saline (PBS), resveratrol and fibrinogen solutions to identify the location, drifting and aggregation of lipid rafts. Via cross-comparison of AFM images, the regions are higher and stiffer than the surrounding cell membranes resemble the characteristics of lipid rafts. The developed method may be applicable to identify the location of lipid rafts in real-time and allow us to elucidate complex biological systems at the molecular level.