SKM 2023 – scientific programme

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SYFP: Symposium Physics of Fluctuating Paths

SYFP 1: Physics of Fluctuating Paths

SYFP 1.2: Invited Talk

Tuesday, March 28, 2023, 10:00–10:30, HSZ 01

Fluctuations and molecule-spanning dynamics of single Hsp90 proteins on timescales from nanoseconds to days — •Thorsten Hugel — Institute of Physical Chemistry, University of Freiburg, Germany — Signalling research centers BIOSS and CIBSS, University of Freiburg, Germany — Cluster of Excellence livMatS @ FIT, University of Freiburg, Germany

Protein dynamics have been investigated on a wide range of timescales. Nano- and picosecond dynamics have been assigned to local fluctuations, while slower dynamics have been attributed to larger conformational changes. However, it is largely unknown how local fluctuations can lead to global changes and on which timescales the proteins dynamics are ergodic and if they show ageing. These questions become even more challenging if ATP hydrolysis is involved.

Here we set out to understand the fluctuations and conformational dynamics in the molecular machine and heat shock protein Hsp90. Therefore, we measure the dynamics of single Hsp90s on timescales from nanoseconds to days with a combination of single-molecule fluorescence, Neutron scattering and Plasmon resonance supported by MD simulations.

I will show that molecule-spanning dynamics on the 100 ns timescale likely precede hierarchical allosteric processes on slower timescales. Then the very good statistic on the even slower conformational dynamics allows us to discuss directionality, ergodicity and ageing in this fascinating ATP fueled molecular machine.