Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 11: Cell Mechanics I

BP 11.6: Vortrag

Dienstag, 19. März 2024, 11:15–11:30, H 2032

Cellular Contraction of Fibroblast-Populated Collagen Gels Reveals Potential Cooperative Cell Behaviors — •Lydia Rebehn¹, Christin Heinrichs¹, Hans Kestler², Karin Scharffetter-Kochanek³, Paul Walther⁴, and Kay-E Gottschalk¹ — ¹Institute for Experimental Physics, Ulm University, Ulm, Germany — ²Institute for Medical Systems Biology, Ulm University, Ulm, Germany — ³Department of Dermatology and Allergology, Ulm University, Ulm, Germany — ⁴Central Facility for Electron Microscopy, Ulm University, Ulm, Germany

Tissue homeostasis is maintained by a delicate balance of mechanical and chemical cues exchanged between the extracellular matrix and the incorporated cells. The mechanical properties of the matrix and the cells themselves cooperate to give tissues their structure and function. To understand the mechanical implications of these cell-matrix interactions we investigate fibroblast contraction in collagen matrices via a 3D printed microscale device [1]. We explore the contraction of fibroblast-populated collagen gels with different cell densities for revealing potential cooperative effects. Furthermore, we use SEM and TEM imaging to expose the impact of cell density on contraction and matrix customization with by the embedded cells. The results highlight the necessity of further investigation into potential collective cell behaviors and the need to explore possible fibroblast transdifferentiation during the experiment. [1] Zhang, Tianzi, et al. "Investigating fibroblast-induced collagen gel contraction using a dynamic Microscale platform". Frontiers in Bioengineering and Biotechnology, vol. 7, 2019

Keywords: cell cooperation; contraction; collagen