Berlin 2024 – scientific programme

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BP: Fachverband Biologische Physik

BP 14: Poster IIa

BP 14.15: Poster

Tuesday, March 19, 2024, 18:00–20:30, Poster E

Noninvasive measurement of tissue tension using low magnification brightfield microscopy — •Mattias Luber, Arne Hofemeier, and Timo Betz — Third Institute of Physics - University of Göttingen, Göttingen, Germany

The interplay of numerous biomechanical features is fundamental for proper tissue function. Therefore, having experimental access to biomechanics is crucial for advancing our understanding of the development of muscle and connective tissue, facilitating the identification of abnormalities and contributing to our understanding of disease mechanisms. Engineered in vitro tissues have proven to function as well-established disease models, allowing mimicking phenotypic conditions in a controlled environment. However, precise measurements of the relevant biomechanical features are still tedious and require specialized equipment as well as manual handling of the sensitive samples. To ease this burden, we present a platform to raise biomimetic tissue models under controlled conditions and measure the tissue tension (among others) noninvasively and throughout the full developmental lifecycle with low magnification brightfield microscopy only. Using this approach, we were able to identify tissue tension as a highly relevant readout for muscular disease like Duchenne Dystrophy, demonstrate the tension-regulating effect of certain compounds or, in context of connective tissues, to investigate fibrotic conditions.

Keywords: Biomechanics; Tissue Tension; Biomimetic Tissue Models