Berlin 2024 – scientific programme
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BP: Fachverband Biologische Physik
BP 14: Poster IIa
BP 14.16: Poster
Tuesday, March 19, 2024, 18:00–20:30, Poster E
Dense optical flow analysis to quantify spatiotemporal fluctuations during tissue growth — •Kai Lennard Fastabend1, Tassilo von Trotha2, Mario Christian Benn2, Viola Vogel2, and Philip Kollmannsberger1 — 1Biomedical Physics, Heinrich-Heine-Universität Düsseldorf, Germany — 2Laboratory of Applied Mechanobiology, ETH Zürich, Switzerland
The growth of fibroblast microtissues depends on the interplay between cell contractility, extracellular matrix and the geometry of the underlying substrate. Previous studies revealed gradients of cell phenotype and matrix stretch between growth front and tissue interior [1], but how these gradients emerge over time is not clear. We aim to better understand the dynamics of growth by quantifying spatiotemporal deformation patterns during growth. We first evaluated different strain mapping algorithms applied to phase contrast time lapse movies of growing tissues from [2] and found that the Farnebäck algorithm for dense optical flow showed the most robust results. Based on the resulting deformation fields, the time dependent divergence of motion was calculated to identify regions of stretching and compression. Spatial and temporal smoothing of the flow fields was employed to evaluate local tissue deformations at different scales. The presented framework is a promising approach to analyze the mechanical feedback regulation involved in the organization of tissue growth, enabling a more nuanced understanding of cellular responses to microenvironmental mechanical cues. [1] P Kollmannsberger et al., Science Advances 4(1) eaao4881 (2018) [2] MC Benn et al. Science Advances 9(13) eadd9275 (2023)
Keywords: tissue growth; microtissues; optical flow