Berlin 2024 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 14: Poster IIa

BP 14.3: Poster

Dienstag, 19. März 2024, 18:00–20:30, Poster E

Drug interactions between antibiotics targeting translation and transcription — •Natawan Gadjisade and Tobias Bollenbach — University of Cologne, Institute for Biological Physics, Germany

Combining antibiotics has the potential to improve treatment efficacy and slow the evolution of resistance. When two antibiotics are combined, their effect on bacterial growth may be stronger or weaker than expected (i.e., synergistic or antagonistic). Recent work has shown that it is often possible to predict such interactions between ribosome-targeting antibiotics using a biophysical model based on bacterial growth laws. Here, we focus on establishing a new model that resolves the interplay between translation and transcription inhibitors. We classify drug interactions by measuring E. coli growth in two-dimensional concentration gradients of the transcription inhibitor rifampicin and several translation inhibitors. Our data indicate different types of interactions. We systematically quantify proteome allocation and individual protein regulation using mass spectrometry-based proteomics measurements optimized for absolute quantification of protein levels. Our preliminary data from single drug treatment with translation inhibitors confirm the known increase in ribosome concentration, whereas rifampicin led to a decrease. Our current aim is to quantify how the cells resolve the conflict of ribosome regulation by performing proteomics measurements in a two-dimensional concentration gradient of both drugs. This work may lead to a deeper understanding of the interplay of transcription and translation.

Keywords: drug interactions; translation inhibitor; transcription inhibitor; bacterial growth laws; proteomics