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BP: Fachverband Biologische Physik
BP 14: Poster IIa
BP 14.5: Poster
Tuesday, March 19, 2024, 18:00–20:30, Poster E
Guiding Escherichia coli biofilm growth with textured surfaces — •Clémentine Ferrari1, Marie-Ly Chapon2, Laurent Pieuchot2, Wei Wang3,4,5, Weiwei Wang3, Nan Ma3,4, and Cécile M. Bidan1 — 1Max Planck Institute of Colloids and Interfaces, Potsdam — 2Université de Haute-Alsace, Mulhouse — 3Helmholtz-Zentrum Hereon, Teltow — 4Freie Universität Berlin, Berlin — 5Jilin University, Changchun
Biofilms are complex 3D biological materials created by bacteria producing a protective matrix against adverse environments. These bacteria adapt their matrix in response to various chemical, biological or physical stimuli. This implies that the materials properties of biofilms can be influenced by the properties of their substrate. This project aims at regulating the growth and organization of biofilms by manipulating the geometry of their environment through structured surfaces. Therefore, we culture E. coli bacteria on agar plates to obtain 3D biofilms. The surface of the agar plate is previously shaped with different PDMS stamps. The patterns on these stamps consist of parallel periodic sinusoidal waves or an array of concave half-spheres. In both cases, different dimensions are tested to determine if there is a critical length range where biofilms respond to these modifications, and how this affect their growth and properties. First results indicate that microtopography can influence biofilm spreading. Quantitative methods based are now being established to characterize and measure these changes. Ultimately, textured surfaces may help to develop oriented biofilm-based materials with anisotropic properties.
Keywords: Bacterial biofilms; Topography; Microscopy; Biomaterial engineering