Berlin 2024 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 14: Poster IIa

BP 14.9: Poster

Dienstag, 19. März 2024, 18:00–20:30, Poster E

Traffic Slowdown by Antibiotics — •Johannes Keisers, Luca Ciandrini, and Phillippe Fuchs — Centre De Structurale Biologe (CBS), Montpellier, France

Bacteria adapt to environmental changes through significant protein expression changes. Despite the complexity of transcription and translation, quantitative growth laws link ribosome allocation and growth. Our focus is on sub-lethal antibiotic effects, reshaping cellular resources. Using a modified TASEP model, we study antibiotic-induced ribosome pausing states, reproducing the second growth law and quantifying active and inactive ribosomes. The approach interprets the ribosome allocation-growth rate relationship under sub-lethal antibiotic doses. It successfully predicts the acceleration of ribosomes under sub-lethal doses of antitbiotics, as observed in E. coli. Our model estimates active versus antibiotic-bound ribosomes and predicts antibiotic impact on charged tRNA concentration. This framework enhances understanding of sub-lethal antibiotic effects on bacterial dynamics, potentially informing future transcriptional perturbations.

Keywords: Non-equilibrium dynamics; Bacterial Growth Laws; Totally Asymmetric Simple Exclusion Process