Berlin 2024 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Poster IIb
BP 15.10: Poster
Dienstag, 19. März 2024, 18:00–20:30, Poster F
Combining fluorescence lifetime imaging and expansion microscopy for investigation of Actin staining approaches — •Elza Sunil1, Shangjun Cheng1,2,3, Subham Adak1, Sara Gjeci1,3, Aleksandar Rusevski1,3, Hans-Dieter Arndt1, Adrian T. Press1,3, Daniela Täuber1,2, and Rainer Heintzmann1,2 — 1Friedrich Schiller University Jena — 2Leibniz Institute of Photonic Technology, Jena — 3Jena University Hospital, Jena, Germany
Cytoskelettal Actin plays an important role in cell stability and mobility. In a previous study, an increased amount of aggregated F-Actin has been found in liver tissue from infected animals in a mouse model for systemic infection [1]. We combine fluorescence lifetime imaging and expansion microscopy to evaluate different Actin staining approaches aiming at enhancing our understanding of Actin aggregation in the context of infection and therapy. [1] P. Martinac, A.T. Press, A. Medyukhina, K.-J. Benecke, J. Shi, D. Täuber, S. Hoeppener, Z. Cseresnyes, I.G. Scheblykin, M.H. Gräler, I. Rubio, M.-T. Figge, U.S. Schubert. M. Bauer: Inhibition of phosphoinositide 3-kinase-y improves liver function in sepsis by preventing RhoA-mediated cholestasis in 9th International Congress "Sepsis and Multiorgan Dysfunction", Infection, 47, S6-S7, 2019.
Keywords: Actin staining; Fluorescence lifetime imaging; expansion microscopy; confocal microscopy