Berlin 2024 – scientific programme
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BP: Fachverband Biologische Physik
BP 15: Poster IIb
BP 15.11: Poster
Tuesday, March 19, 2024, 18:00–20:30, Poster F
Synchronisation of confocal laser scanning and single photon counting in a homebuilt Fluorescence lifetime imaging microscopy (FLIM) setup — •Subham Adak1,2,3, Elza Sunil1,2, Monalisa Goswami1,2, Daniela Täuber1,2, and Rainer Heintzmann1,2,3 — 1Leibniz Institute of Photonic Technology, Jena — 2Institute of Chemical Physics, Friedrich Schiller University Jena — 3Abbe Center of Photonics, Jena, Germany
Fluorescence Lifetime Imaging Microscopy (FLIM) is an attractive microscopy method in the life sciences, yielding information on the sample otherwise unavailable through intensity-based techniques [1]. In our homebuilt FLIM setup we combine a confocal laser scanning system from LaVison BioTec with a single photon counting system from picoQuant. The triggers for confocal scanning and for single photon acquisition are carefully synchronized. We tested (i) the accuracy of the determined lifetimes, and (ii) the time and spatial resolution of the instrument using fluorescent beads of different diameters. [1] A. Le Marois, S. Labouesse, K. Suhling, and R. Heintzmann, (2017), Noise-Corrected Principal Component Analysis of fluorescence lifetime imaging data. J. Biophoton., 10: 1124-1133.
Keywords: fluorescence lifetime imaging microscopy; confocal laser scanning; single photon counting