Berlin 2024 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 15: Poster IIb

BP 15.12: Poster

Dienstag, 19. März 2024, 18:00–20:30, Poster F

Oxygen Measurements of single Red Blood Cells by Lightmicrospy — •Sarah Tabea Hermes, Agatha Belen Pinto Pino, Thomas John, and Christian Wagner — Campus E2.6 66123 Saarbrücken

The red blood cells in our body have a very high affinity for absorbing oxygen. In the lungs, they are loaded with oxygen and release it again in the body. The release of oxygen changes the light spectrum of the hemoglobin, thereby slightly altering its color. At selected wavelengths, this difference is significant and can be utilized to detect the O₂ content of individual cells using light microscopy. We are discussing various methods to prepare red blood cells in vitro without O₂. The objective is to investigate the oxygen release or uptake of individual cells under microfluidic conditions.

Keywords: Red blood cells; Oxygenation state; microfluidics; mircoscopy