Berlin 2024 – scientific programme

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BP: Fachverband Biologische Physik

BP 15: Poster IIb

BP 15.19: Poster

Tuesday, March 19, 2024, 18:00–20:30, Poster F

Visualizing Molecular Dynamics with High-Speed Tip-Scanning Atomic Force Microscopy — •Jörg BArner, Andre Körnig, Thomas Henze, and Heiko Haschke — JPK BioAFM Business, Bruker Nano GmbH, Am Studio 2D, 12489 Berlin, Germany

Biological systems exhibit very high structural and functional dynamics on molecular scales. Understanding the principles of the kinetics behind structural changes at that scale is of critical importance when studying samples ranging from single membrane proteins to complex macromolecular systems, in order to accurately develop novel therapeutic applications. We have used high-speed tip-scanning atomic force microscopy (AFM) with a kilohertz line-rate to visualize molecular dynamics by enabling temporal resolution on the sub-100-milisecond scale. The use of a tip-scanning AFM, as compared to a sample-scanning system, enables high-resolution correlation experiments with advanced optical techniques. We will give two examples in which high-speed tip-scanning AFM was applied for studying of structural transitions and biomolecular dynamics in samples, containing triangular DNA origamis and photosensitive azobenzene-containing surfactants.

Keywords: AFM; High-Speed; Microscopy; DNA origami; CIS-TRANS isomisation