Berlin 2024 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 15: Poster IIb

BP 15.20: Poster

Dienstag, 19. März 2024, 18:00–20:30, Poster F

Virtual 3D Histology using Synchrotron Radiation: Present Status of GINIX, Outlook to PETRA IV — •Markus Osterhoff and Tim Salditt — Institut für Röntgenphysik, Uni Göttingen, Göttingen

We present the current state of the art of Synchrotron based phase-contrast tomography in a multi-scale setup.

In waveguide-filtered cone-beam geometry, high resolution scans (voxel sizes reaching 100 nm) reveal minute details of the specimen, at the scale of organelles. Conversely, the parallel beam geometry facilitates rapid acquisition, capturing a larger field of view, thereby integrating sub-cellular data within a broader physiological context. Thus, tomography scans with voxel sizes of 650 nm achieve a 2D in-plane resolution comparable to microscopic histology. However, as a non-invasive, fully three-dimensional technique, it eliminates the need for physical slicing of samples, resulting in isotropic 3D resolution.

We could image human lung tissue severely affected by Covid-19, and evidence diffuse alveolar damage with its prominent hyaline membrane formation. Extending conventional histopathological examination by a third dimension allows to analyse the delicate pathological changes of the vascular system of severe Covid-19 progressions.

In the future, with GINIX II we are aiming to improve biomedical x-ray tomography at better spatial resolution under physiological conditions. Notably, automated measurements and real-time analysis of raw detector images ensures balance between speed and accuracy for clinical diagnostics and research applications.

Keywords: X-ray Tomography; Synchrotron Radiation; Virtual Histology