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BP: Fachverband Biologische Physik
BP 15: Poster IIb
BP 15.2: Poster
Dienstag, 19. März 2024, 18:00–20:30, Poster F
High-resolution chemical imaging of model system Bacillus subtilis using mid-IR photo-induced force microscopy (PiF-IR) — •Selema Buzhala1,2, Robin Schneider1, Maryam Ali2, Astrid Tannert1,3, Sebastian Unger1,2, Rainer Heintzmann1,2, Ute Neugebauer1,2,3, and Daniela Täuber1,2 — 1Leibniz Institute of Photonic Technology, Jena — 2Friedrich Schiller University Jena — 3Jena University Hospital, Center for Sepsis Control and Care, Jena, Germany
Mid-infrared photo-induced force microscopy (PiF-IR) offers high spectral resolution in combination with surface sensitivity and a spatial resolution in the range of a few nanometers. In a recent study, we demonstrated its ability to reveal local variations in the secondary protein structure of F-Actin on a scale of 5 nm [1]. Here we apply PiF-IR to individual cells of the well-known Bacillus subtilis treated with an antibacterial drug and to untreated controls. Cropped scans at high spatial resolution visualize variations in the sugar and peptide contents of the bacterial cell walls. Additional chemical information is provided from the analysis of hyperspectral images using home-written software. [1] J. Joseph, L. Spantzel, M. Ali, D.M. Joseph, S. Unger, K. Reglinski, C. Krafft, A.-D. Müller, C. Eggeling, R. Heintzmann, M. Börsch, A.T. Press, D. Täuber. Nanoscale chemical characterization of secondary protein structure of F-Actin using mid-infrared photoinduced force microscopy (PiF-IR). Spectrochimica Acta part A: Molecular and Biomolecular Spectroscopy, 306, 123612, 2024.
Keywords: Infrared spectroscopic imaging; mid-IR photo-induced force microscopy; Bacillus subtilis; Nanoscale resolution; chemical characterization