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BP: Fachverband Biologische Physik

BP 15: Poster IIb

BP 15.3: Poster

Dienstag, 19. März 2024, 18:00–20:30, Poster F

Robust and fast sorting of droplets in microfluidic devices by droplet size and droplet content based on bright field and fluorescent information — •Jonas Pfeil^1,2, Patricia Schwilling¹, and Othmar Marti¹ — ¹Universität Ulm, Ulm, Deutschland — ²Sensific GmbH, Biberach, Deutschland

Droplet-based microfluidics is a promising tool to manipulate biological systems in small sample volumes down to single-cell level. Active sorting of droplets allows to enrich target configurations with high selectivity and selectivity.

We present methods and tools required to enrich droplets based on size and content in multiplexed bright-field and fluorescent microscopic imaging at sorting rates of 60 Hz. The PDMS-based microfluidic device uses a two electrode design with an high voltage AC field to sort droplets via dielectrophoretic forces.

Keywords: Microfluidics; Droplet; Sorting; Fluorescence