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BP: Fachverband Biologische Physik
BP 15: Poster IIb
BP 15.9: Poster
Tuesday, March 19, 2024, 18:00–20:30, Poster F
In vivo Actin staining approaches validated using structured illumination and expansion microscopy — •Shangjun Cheng1,2,3, Sara Gjeci1,3, Aleksandar Rusevski1,3, Patrick Then1,4, Hans-Dieter Arndt1, Rainer Heintzmann1,2, Daniela Täuber1,2, and Adrian T. Press1,3 — 1Friedrich Schiller University Jena — 2Leibniz Institute of Photonic Technology, Jena — 3Jena University Hospital, Jena — 4Microverse Imaging Center, Jena, Germany
Actin assembly and disassembly is essential for any type of cell mobility. Variations in Actin content in liver tissue have been found to be an indicator for animal survival in a recent study utilizing a mouse model for systemic infection [1]. In vivo imaging of Actin, thus, provides access to enhanced understanding of cellular behavior in various areas of research including the response to infection and therapy. We use structured illumination and expansion microscopy to evaluate several approaches for in vivo Actin staining. [1] P. Martinac, A.T. Press, A. Medyukhina, K.-J. Benecke, J. Shi, D. Täuber, S. Hoeppener, Z. Cseresnyes, I.G. Scheblykin, M.H. Gräler, I. Rubio, M.-T. Figge, U.S. Schubert. M. Bauer: Inhibition of phosphoinositide 3-kinase-y improves liver function in sepsis by preventing RhoA-mediated cholestasis in 9th International Congress "Sepsis and Multiorgan Dysfunction", Infection, 47, S6-S7, 2019.
Keywords: structured illumination microscopy; Actin staining; in vivo; expansion microscopy