Berlin 2024 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 16: Membranes and Vesicles II
BP 16.10: Vortrag
Mittwoch, 20. März 2024, 12:00–12:15, H 0112
Application of Homogenization Techniques to Gas-Phase Deposited DPPC Films on Silicon Substrates: Unveiling Phase Transitions in Dry Environments of DPPC Bilayers — •Nicolás Moraga1, Daniel Saavedra1, Nancy Gomez-Vierling1, Marcelo A. Cisternas2, María José José Retamal3, and Ulrich G. Volkmann1 — 1Instituto de Física, Pontificia Universidad Católica de Chile, Santiago, Chile — 2Escuela de Ingeniería Industrial, Universidad de Valparaíso, Chile — 3Facultad de Ingeniería, Universidad Finis Terrae, Santiago, Chile
The study investigates homogenization techniques for dipalmitoylphosphatidylcholine (DPPC) phospholipid bilayers deposited on single crystal silicon substrates through vapor phase deposition. Initial deposition employed physical vapor deposition (PVD) with controlled thickness and rate. Subsequent annealing, conducted in varied environments (in air and dry N2 at STP, vacuum), and temperature ramps in a dry N2 atmosphere (different pressures up to 1000 Torr) were explored as homogenization methods. The research emphasizes the critical role of precise and reproducible deposition rates during annealing in achieving homogeneity in DPPC bilayers. Different annealing conditions led to diverse effects on homogeneity, indicating distinct outcomes in air, nitrogen environments at different pressures, and high vacuum. The study revealed not only topographical changes but also documented phase transitions, suggesting the formation of lipid bilayers even in dry environments without the need for hydration. Acknowledgements: ANID Fellowships (NM, DS, NGV).
Keywords: Homogenization of dry SLBs; Artificial Membrane growth in High Vacuum; Phase Transitions of dry phospholipid bilayers; Ellipsometric in situ Thickness Control; Non hydrated Biomimetic Films