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BP: Fachverband Biologische Physik
BP 16: Membranes and Vesicles II
BP 16.8: Vortrag
Mittwoch, 20. März 2024, 11:30–11:45, H 0112
Quantification of mRNA and siRNA content of Lipid Nanoparticles — •Bernhard Kirchmair1, Judith Müller1, Thomas Kellerer2, and Joachim Rädler1 — 1Ludwig-Maximilians-Universität München — 2Hochschule München
Lipid Nanoparticles (LNPs) have been proven to be promising vectors to deliver mRNA to mammalian cells. Advanced strategies using multi-component nucleic acid motifs require a reliable quantification of the stoichiometric ratios. This project seeks to quantify the mRNA-content when varying the LNP size and surface composition. Employing Fluorescence Correlation Spectroscopy (FCS) measurements, assisted by Dynamic Light Scattering (DLS), both size and concentration of LNPs in solution can be estimated, allowing to obtain the average number of mRNA strands per particle. Based on this, using Fluorescence Cross Correlation, the stoichiometric ratio of short interfering RNA (siRNA) and mRNA, both fluorescently labeled, will be determined. Controlled co-formulation of siRNA and mRNA is expected to allow for regulated mRNA expression.
Keywords: Lipid Nanoparticles; Fluorescence correlation spectroscopy; mRNA; LNP; FCS