Berlin 2024 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 16: Membranes and Vesicles II
BP 16.9: Vortrag
Mittwoch, 20. März 2024, 11:45–12:00, H 0112
Interplay of phospholipids and saponins - why the application of complementary techniques is important — •Carina Dargel1,2, Friederike Gräbitz-Bräuer2, Lionel Porcar3, and Thomas Hellweg2 — 1University of Münster, Institute of Physical Chemistry, Münster, Germany — 2University of Bielefeld, Physical and Biophysical Chemistry, Bielefeld, Germany — 3Institut Laue-Langevin (ILL), Grenoble, France
Scattering methods are a common tool to analyze structural changes in systems comprising, e.g., phospholipids mixed with natural surfactants such as saponins. Small-angle X-ray and neutron scattering (SAXS and SANS) have been extensively used to study the interaction of the saponins glycyrrhizin and aescin with the phospholipid 1,2-dioleoyl-sn-glycero-3-phosphoglycerol (DOPG), which carries a negatively charged head group. While for a small unilamellar vesicle (SUV) system prepared from the zwitterionic lipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), membrane solubilization and thus bicelle formation was observed upon saponin addition[1], hardly any interaction could be detected for the DOPG-saponin mixtures[2,3]. Instead, DOPG SUVs coexist with saponin micelles/monomers. The investigated system clearly demonstrates the importance of using complementary techniques such as SAXS and SANS to avoid misleading conclusions from only a single method.
[1] Geisler et al. (2019), Molecules, 25(1), 117.; [2] Dargel et al. (2021), Molecules, 26(16), 4959; [3] Gräbitz-Bräuer & Dargel et al. (2023), Colloid and Polymer Science, 1-14
Keywords: phospholipid; saponin; small-angle scattering; SAXS; SANS