Berlin 2024 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Bioimaging
BP 17.3: Vortrag
Mittwoch, 20. März 2024, 10:00–10:15, H 2032
The behaviour of vital mitochondria in response to nanoprobing using Scanning Ion Conductance Microscopy (SICM) — •Eric Lieberwirth1, Christian Völkner1, Regina Lange1, Anja Schaeper2, Magdalena Otte2, Rica Waterstradt2, Annett Kott2, Ingo Barke1, Simone Baltrusch2, and Sylvia Speller1 — 1University of Rostock, Institute of Physics — 2Rostock University Medical Center, Institute of Medical Biochemistry and Molecular Biology
The mitochondrial network maintains contacts with cell organelles such as the peroxisome, endoplasmic reticulum (ER) or cytoskeleton, but also undergoes constant remodelling of its structure and shape. In order to understand the processes further, we examine vital, metabolizing mitochondria extracted from HeLa cells using the non-contact Scanning Ion Conductance Microscope (SICM). The lateral resolution is approx. 50 nm - 100 nm and the resolution in the z-direction is approx. 1 nm. Besides the known diversity of shapes of the mitochondrial network, simple image processing techniques are sufficient to visualize structures, compatible with cristae folds. We observed a peculiar effect at the edges of mitochondria which decreases with time and which we interpret as a signature of vitality. An attempt of interpretation is based on the mistaking of the glass nanopipette probe as an organelle, e. g. as ER or microtubule.
Keywords: SICM; mitochondria; vital; isolated; HeLa