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BP: Fachverband Biologische Physik
BP 17: Bioimaging
BP 17.4: Vortrag
Mittwoch, 20. März 2024, 10:15–10:30, H 2032
High-resolution chemical imaging in mid-infrared photo-induced force microscopy (PiF-IR) — •Maryam Ali1, Selema Buzhala1,2, Sebastian Unger1,2, Christoph Krafft1,2, Rainer Heintzmann1,2, and Daniela Täuber1,2 — 1Friedrich Schiller University, Jena — 2Leibniz Institute of Photonic Technology, Jena, Germany
Non-contact force microscopy is able to report changes in the attractive Van-der-Waals (VdW) force between a metallic tip and a sample. In mid-IR photo-induced force microcopy (PiF-IR), thermal expansion due to absorption leads to a change in the VdW force, which is reported via a heterodyne detection scheme. This results in an unprecedented resolution < 5 nm for chemical imaging of surfaces including bacteria walls and cellular tissue[1]. In biomaterials, important chemical variations have to be identified above a rather heterogeneous background. Topological Data Analysis (TDA) is a promising approach for extracting signals of interests in hyperspectral PiF-IR images. We compare the analysis using TDA with other approaches including Principal Component Analysis (PCA), Hierarchical Clustering Analysis (HCA) and to a guided approach using known spectral components. [1] J. Joseph, L. Spantzel, M. Ali, D. Moonnukandathil Joseph, S. Unger, K. Reglinski, C. Krafft, A.-D. Müller, C. Eggeling, R. Heintzmann, et al., Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2024, 306, 123612.
Keywords: Mid-IR photo-induced force microcopy (PiF-IR); Single bacteria wall; Sub-cellular resolution; High-resolution chemical imaging; Advanced Component Analysis