Berlin 2024 – scientific programme
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BP: Fachverband Biologische Physik
BP 18: Biomaterials and Biopolymers (joint session BP/CPP)
BP 18.7: Talk
Wednesday, March 20, 2024, 11:30–11:45, H 1028
Dynamic DNA origami nanopores — •Anna Baptist1, Ze Yu2, Sabina Caneva2, and Amelie Heuer-Jungemann1 — 1MPI of Biochemistry, Martinsried, Germany — 2TU Delft, The Netherlands
Nanopores are nanoscale structures that form channels across membranes and enable the translocation of molecules. Inspired by naturally occurring pore-forming proteins, different types of artificial nanopores have been created. The DNA origami technique allows for the fabrication of DNA nanostructures with precise control over shape and size that can be modified with a variety of functional molecules. Thus, DNA origami provides a platform for the customized design of nanoscale pores with different channel diameters that can be equipped with anchoring molecules for insertion into lipid membranes. Such nanopores have potential applications in single-molecule sensing, sorting of molecules depending on their sizes or for the fabrication of artificial cells. However, most nanopores created so far are static with a fixed pore diameter. Here, we present a large dynamic DNA origami nanopore that can be mechanically and reversibly switched between different conformations via strand displacement, offering three different pore sizes. After their successful insertion into the lipid bilayer, these nanopores form transmembrane channels with varying diameters depending on their conformation and can be used to control the transport of differently sized molecules across the lipid membrane. Such stimuli-responsive, actuatable nanopores are excellent mimics of complex natural occurring pores, while enabling a higher level of control and a more modular and easily adaptable design.
Keywords: DNA origami; nanopore