Berlin 2024 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 2: Membranes and Vesicles I
BP 2.6: Vortrag
Montag, 18. März 2024, 10:45–11:00, H 2032
Mechanical regulation of endocytosis by protein condensate capillary forces — •Max Ferrin1,2,3, Tyler Harmon2, David Drubin3, and Frank Jülicher1 — 1Max Planck Institute for the Physics of Complex Systems, Dresden, Germany — 2Institute Theory of Polymers, Leibniz Institute of Polymer Research, Dresden, Germany — 3Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
Clathrin-mediated endocytosis (CME) is the primary pathway for internalization of extracellular and membrane cargo in eukaryotic cells. It is characterized by a patch of the plasma membrane invaginating and pinching off to generate a cytoplasmic vesicle. Recently, experimental evidence has begun to accumulate in support of proteins assembling into liquid-like condensates at nascent CME sites, but potential functional consequences are lacking in the literature. Given that condensate capillary forces influence membrane bending in other biological systems, we constructed a mathematical model to probe the mechanical influence of a protein droplet on endocytosis. Preliminary analysis of the model shows that a droplet can regulate the progression of endocytosis by stalling invagination until a threshold distance or droplet volume, and driving toward completion after passing the threshold. Further model analysis will characterize the critical parameters that set this regulatory behavior, as well as make predictions of CME dynamics that can be tested experimentally.
Keywords: endocytosis; biomolecular condensation; thermodynamic model; capillarity; membrane mehcanics