Berlin 2024 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 20: Poster IIIa
BP 20.35: Poster
Mittwoch, 20. März 2024, 11:00–14:30, Poster B
Periodic impulse response of ERK signaling in HeLa cells — •Stefan Köstler1, Thoma Itoh2, and Kazuhiro Aoki2 — 1Max Planck Institute for Dynamics and Self-Organization, Göttingen, Germany — 2National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Japan
The Extracellular-signal Regulated Kinase (ERK) signaling pathway is one of the most important signaling pathways controlling the cell cycle and survival of most cells. Defects in this pathway are a critical step in the development of many cancers, making it a valuable target for treatment strategies. To gain insight into its response behavior, we activate ERK from different layers of the pathway by impulse stimulation. The ERK activity is quantified by using the fluorescent biosensor ERK-KTR-iRFP, which is exported from the nucleus upon phosphorylation. We show that impulse stimulation of two different layers of the pathway, either by EGF or the optogenetic tool Opto-RAF, both lead to a repeated activation of ERK with a characteristic period of about 65 minutes.
Keywords: signaling pathway; cell; optogenetics; signaling; fluorescent imaging