Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 21: Poster IIIb

BP 21.22: Poster

Mittwoch, 20. März 2024, 11:00–14:30, Poster C

A NAP-XPS-study on X-ray radiation damage: Chemical changes to Gene-V Protein — •Dorothea C. Hallier^{1, 2, 3}, Jörg Radnik², Paul M. Dietrich⁴, Harald Seitz^{1, 3}, and Marc Benjamin Hahn² — ¹Fraunhofer Insitute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany — ²Federal Insitute for Materials Research and Testing BAM Berlin, Berlin, Germany — ³Univerity of Potsdam, Institute for Biochemistry and Biology, Potsdam Germany — ⁴SPECS Surface Nano Analysis GmbH, Berlin, Germany

Single-stranded DNA-binding proteins such as Gene-V Protein (G5P/GVP) are involved in maintaining the DNA metabolism in cells. This is essential for cell viability, especially after exposure to ionizing radiation, i.e. after radiation therapy in cancer treatment. X-ray photoelectron spectroscopy (XPS) was used to analyze the chemical damage of ionizing radiation to G5P itself. Direct and indirect damage was detected through combined vacuum XPS and near-ambient pressure (NAP) XPS measurements under water atmosphere. A strong increase of protein damage was observed in water as compared to vacuum.

Keywords: radiation damage; X-ray; ssDNA-binding protein; XPS; NAP XPS