Berlin 2024 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 27: Single Molecule Biophysics
BP 27.3: Vortrag
Donnerstag, 21. März 2024, 10:00–10:15, H 0112
Label-free imaging and 3D single particle tracking in complex media via interferometric scattering microscopy (iSCAT) — •Kiarash Kasaian1,2, Mahdi Mazaheri1,2, and Vahid Sandoghdar1,2 — 1Max Planck Institute for the Science of Light, Erlangen, Germany — 2FAU Erlangen-Nürnberg, Erlangen, Germany
Label-free imaging offers a distinct advantage over techniques employing fluorescent labels by eliminating concerns related to photobleaching and photo-toxicity. iSCAT is a highly sensitive tool for label-free microscopy [1]. However, imaging complex biological specimens with iSCAT remains challenging, as the scattering from multiple sources generates dynamic speckle patterns, which can obscure the signal of interest. Introducing a novel iSCAT modality termed "diffused-illumination iSCAT" (DI-iSCAT) [2], we engineer the spatial coherence of the illumination to mitigate the impact of dynamic speckle. We demonstrate high-speed imaging of live cells over a large field of view of 100 µ m × 100 µ m and employ our newly developed 3D tracking algorithm [3] to perform 3D tracking of nanoparticles in cellular environment.
[1] Taylor, R. W.; et al. Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane. Nat. Photonics 13, 480 (2019)
[2] Mazaheri, M.; et al. Label-Free Imaging and Tracking with Speckle-Free Interferometric Scattering Microscopy (in preparation)
[3] Kasaian, K.; et al. Long-Range High-Speed 3D Tracking via Interferometric Scattering Microscopy (in preparation)
Keywords: label-free imaging; interferometric scattering microscopy; 3D tracking