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BP: Fachverband Biologische Physik

BP 31: Protein Structure and Dynamics

BP 31.4: Invited Talk

Thursday, March 21, 2024, 15:45–16:15, H 0112

Polarizing nuclear spins at the interface between ESR and NMR spectroscopy — •Marina Bennati — Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany — Institute of Physical Chemistry, University of Göttingen, Göttingen, Germany

Latest developments in magnetic resonance spectroscopy are aimed at increasing sensitivity for nuclear spin detection, which is limited by the small energy splitting at the available polarizing magnetic fields. A powerful approach is taking advantage of the larger magnetic moment of unpaired electrons and uses hyperfine couplings to transfer their polarization to nuclear spins.

The talk will illustrate recent progress in polarization transfer experiments using electron-nuclear double resonance techniques, either with ESR or NMR detection. In ESR spectroscopy, we detect nuclear spins up to the second ligation sphere of a paramagnetic center. We have recently demonstrated that at high electron Larmor frequencies (263 GHz) nuclear chemical shift tensors as well as quadrupolar nuclei can be resolved. In conjunction with 19F spin labelling, our techniques can be employed for measuring inter-spin distances in biomolecules in the angstrom to nanometer range. Moreover, we are developing dynamic nuclear polarization for enhanced NMR detection in the liquid state. Progress and strategies for future applications will be discussed.

Keywords: Nuclear Spins; Hyperfine Couplings; Polarization Transfer